Adi Salzberg, DSc
Associate Professor of Genetics
DSc, 1992 - Technion, Israel
Cell fate diversification in the Drosophila chordotonal organ lineage
One of the fascinating questions in developmental biology is how different cells, which develop within a given organ, acquire different properties that allow them to work together as a functional unit. We are using the proprioceptive organs in the peripheral nervous system (PNS) of Drosophila (also called chordotonal organs, ChOs) as a model system for studying cell fate diversification. The fly ChOs consist of five lineage-related cell types with distinct identities and properties. Very little is known about the mechanisms regulating the specification of the various ChO cell identities. We have constructed a unique transgenic fly strain in which different ChO cell types are labeled with different fluorescent reporters. This strain is used as the basis for large-scale RNAi-based F1 screens for novel determinants of cell fate specification in the ChO lineage. In addition this strain allows us, for the first time, to study post-embryonic development and morphogenesis of ChOs.
Nachman A, Halachmi N, Matia N, Manzur D, and Salzberg A. 2015. Deconstructing the complexity of regulating common properties in different cell types: lessons from the delilah gene. Dev Biol. 403, 180-191.
Zohar-Stoopel A*, Drucker-Gonen N*, Mahroum M, Ben-Zvi DS, Toledano H and Salzberg A 2014. Homothorax plays autonomous and non-autonomous roles in proximodistal axis formation and migration of the Drosophila renal tubules. Dev Dyn. 243, 132-144. * These authors contributed equally to the work.
Pechkovsky A, Lahav M, Bitman E, Salzberg A *, and Kleinberger T.* 2013. E4orf4 induces PP2A- and Src-dependent cell-death in Drosophila melanogaster and at the same time inhibits classical apoptosis pathways. Proc Natl Acad Sci USA 110, E1724-33. *Co-corresponding authors.
Halachmi N, Nachman A, and Salzberg A. 2012. Visualization of proprioceptors in Drosophila larvae and pupae. J. Vis. Exp. 64, e3846.
Egoz-Matia N, Nachman A, Halachmi N, Toder M, Klein Y, and Salzberg A. 2011. Spatial regulation of cell adhesion in the Drosophila wing is mediated by Delilah, a potent activator of βPS integrin expression. Dev Biol. 351, 99-109.
Visualizing the chordotonal organs of a fly embryo.
A lateral view of four abdominal segments of a fly embryo carrying a chordotonal-specific reporter (deiChO-lacZ stained in red), stained for the chordotonal specific α-tubulin 85E (blue) and for the neuronal marker ELAV (green). Eight chordotonal organs are present in each segment: the ventral VChA and VChB, the lateral LCh1 organ and five organs clustered in the LCh5 organ. The cap-attachment (CA), cap cells (C), neurons (N) and ligament cells (L) of the LCh5 organs are denoted.